Identification of pork (susscrofa), horse (Equuscaballus) and donkey (Equusasinus) by PCR-RFLP-DHPLC analysis on the mitochondrial COI gene
نویسندگان
چکیده
Donkey flesh is a popular food in China. To ensure the quality of donkey products plays a vital role in protecting the interests of consumers. Pork and horse are potential in donkey flesh food because of their easier availability at cheaper prices. In present study, PCR-RFLP-DHPLC technology was established for distinguishing the pork, horseflesh and donkey flesh in donkey flesh food. The assay combined the species-specific primers to selectively amplify a short fragment of mitochondrial cytochrome c oxidase subunit I (COI) gene from a heterogeneous background of genomic DNAs followed by RFLP-DHPLC analysis. The assay was sensitive enough to detect 5ng PCR products and finally 7 of marked donkey products were contaminated in 60 samples. Besides pork and horse, no other animal derived ingredients were blended in donkey in these samples.This research brings a promising way to rapid detect any contamination in a specific flesh food.
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